Part:BBa_K3058000:Design

Uricase from Cyberlindnera jadinii

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 510
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 532
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

To avoid codon-bias, the yeast URI gene is codon-optimized for better protein expression in E. coli.

Source

Sequence of URI gene in Cyberlindnera jadinii was codon-optimized by IDT's software and synthesized by IDT.